Kaohsiung Journal of Medical Sciences
Volume 24, Issue 2 , Pages 55-62, February 2008

Phylogenetic Study of Dengue-3 Virus in Taiwan with Sequence Analysis of the Core Gene

  • Yi-Ching Tung

      Affiliations

    • Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
    • School of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
    • ,
  • Kuei-Hsiang Lin

      Affiliations

    • School of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
    • Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
    • ,
  • Ko Chang

      Affiliations

    • Department of Internal Medicine, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung, Taiwan
    • ,
  • Liang-Yin Ke

      Affiliations

    • Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
    • Department of Laboratory Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
    • ,
  • Guan-Ming Ke

      Affiliations

    • School of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
    • ,
  • Po-Liang Lu

      Affiliations

    • Graduate Institute of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
    • School of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
    • Division of Infectious Disease, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
    • Corresponding Author InformationAddress correspondence and reprint requests to: Dr Po-Liang Lu, Department of Internal Medicine, Kaohsiung Medical University Hospital, 100 Tzyou 1st Road, Kaohsiung 807, Taiwan
    • ,
  • Chun-Yu Lin

      Affiliations

    • Division of Infectious Disease, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
    • ,
  • Yen-Hsu Chen

      Affiliations

    • School of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
    • Division of Infectious Disease, Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
    • ,
  • Horn-Che Chiang

      Affiliations

    • School of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

Received 22 May 2007; accepted 29 August 2007.

Article Outline

Dengue virus serotype 3 (dengue-3) has been classified into five genotypes (I–V) by phylogenetic analysis based on different viral genes. To investigate the genetic variability and evolutionary character of the dengue-3 isolates in southern Taiwan from 2005 to 2006, we analyzed the 290 nucleotides of the core (C) gene of 12 dengue-3 isolates and compared them with the published C gene sequences of global dengue-3 strains available in GenBank, including four isolates from 1998 and one isolate from 1999, from Taiwan. The dengue-3 viruses from 2005 to 2006 were not from continuous spread of an epidemic strain or re-emergence of the 2005 strains in the 2-year period because there was a 5.4–6.2% difference in the 290 nucleotides of the C gene and different genotypes between the 2005 and 2006 strains. Most of the nucleotide changes, compared with a prototype dengue-3 virus, H87, occurred in the third codon position and were non-synonymous mutations occurring naturally in the C gene. In addition, there was no consistent difference in the 290 nucleotides of the C gene between eight dengue fever and two dengue hemorrhagic fever isolates from 2006. The phylogenetic analysis indicates that the isolates from the 1998, 1999 and 2006 Taiwan dengue-3 epidemics are phylogenetically related and belong to genotype III. It was noted that the 2005 Taiwan dengue-3 isolates belong to another genotype. This molecular epidemiology study of dengue-3 viruses in Taiwan helps to elucidate whether there is a continuation of outbreaks in consecutive years, re-emergence of endemic dengue virus, or introduction of strains from other countries.

Key Words:  core gene , dengue-3 virus , phylogenetic analysis

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PII: S1607-551X(08)70098-6

doi:10.1016/S1607-551X(08)70098-6

Kaohsiung Journal of Medical Sciences
Volume 24, Issue 2 , Pages 55-62, February 2008

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