Kaohsiung Journal of Medical Sciences
Volume 24, Issue 5 , Pages 254-261, May 2008

Endothelin-1 Enhances Corneal Fibronectin Deposition and Promotes Corneal Epithelial Wound Healing after Photorefractive Keratectomy in Rabbits

  • Yu-Hung Lai

      Affiliations

    • Department of Ophthalmology, Kaohsiung Medical University Hospital and Kaohsiung Medical University, Changhua, Taiwan
    • ,
  • Hwei-Zu Wang

      Affiliations

    • Department of Ophthalmology, Kaohsiung Medical University Hospital and Kaohsiung Medical University, Changhua, Taiwan
    • Department of Ophthalmology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Changhua, Taiwan
    • ,
  • Chang-Ping Lin

      Affiliations

    • Department of Ophthalmology, Changhua Christian Hospital, Changhua, Taiwan
    • Corresponding Author InformationAddress correspondence and reprint requests to: Dr Chang-Ping Lin, Department of Ophthalmology, Changhua Christian Hospital, 135 Nanhsiao Street, Changhua 500, Taiwan
    • ,
  • Show-Jen Hong

      Affiliations

    • Department of Pharmacology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Changhua, Taiwan
    • ,
  • Shun-Jen Chang

      Affiliations

    • Department of Public Health, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Changhua, Taiwan

Received 17 September 2007; accepted 16 January 2008.

Article Outline

The objective was to study the effects of endothelin-1 (ET1) on corneal wound healing after photorefractive keratectomy (PRK) in rabbit corneas. Following PRK, 18 New Zealand white rabbits were treated with ET1 in the right eyes and with phosphate-buffered salt solution (PBS) in the left eyes. Corneal epithelial wound size, corneal haze and corneal thickness were recorded. Corneal extracellular matrixes, including collagen types 3, 4 and 7, chondroitin sulfate and fibronectin, were investigated using immunohistochemistry study. ET1 increased the rate of healing of corneal epithelial wounds in rabbits. Anti-fibronectin fluorescence was present at week 12 and week 24 in ET1-treated eyes but not in the control eyes. There were no significant differences in corneal haze, corneal thickness and changes in other extracellular matrixes between ET1- and PBS-treated eyes. ET1 can enhance the deposition of fibronectin in corneal stroma and promote corneal epithelial wound healing after PRK. The increase in fibronectin probably explains the increased healing rate of corneal epithelial wounds.

Key Words:  corneal wound healing , endothelin-1 , extracellular matrix , fibronectin , photorefractive keratectomy

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PII: S1607-551X(08)70150-5

doi:10.1016/S1607-551X(08)70150-5

Kaohsiung Journal of Medical Sciences
Volume 24, Issue 5 , Pages 254-261, May 2008

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