Kaohsiung Journal of Medical Sciences
Volume 24, Issue 8 , Pages 398-407, August 2008

Molecular Epidemiology of Dengue Virus Serotype 2 in the Taiwan 2002 Outbreak With Envelope Gene and Nonstructural Protein 1 Gene Analysis

  • Yi-Ching Tung

      Affiliations

    • Department of Public Health, Kaohsiung, Taiwan
    • Graduate Institute of Medicine, Kaohsiung, Taiwan
    • ,
  • Kuei-Hsiang Lin

      Affiliations

    • Department of Clinical Laboratory, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
    • Department of Laboratory Medicine, Kaohsiung, Taiwan
    • ,
  • Hung-Che Chiang

      Affiliations

    • Department of Public Health, Kaohsiung, Taiwan
    • ,
  • Liang-Yin Ke

      Affiliations

    • Graduate Institute of Medicine, Kaohsiung, Taiwan
    • Department of Laboratory Medicine, Kaohsiung, Taiwan
    • ,
  • Yen-Hsu Chen

      Affiliations

    • Graduate Institute of Medicine, Kaohsiung, Taiwan
    • Department of Laboratory Medicine, Kaohsiung, Taiwan
    • Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
    • ,
  • Guan-Ming Ke

      Affiliations

    • Department of Laboratory Medicine, Kaohsiung, Taiwan
    • ,
  • Tun-Chieh Chen

      Affiliations

    • Graduate Institute of Medicine, Kaohsiung, Taiwan
    • Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
    • ,
  • Lee-Chiu Chou

      Affiliations

    • Department of Laboratory Medicine, Kaohsiung, Taiwan
    • ,
  • Po-Liang Lu

      Affiliations

    • Graduate Institute of Medicine, Kaohsiung, Taiwan
    • Department of Internal Medicine, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan
    • Corresponding Author InformationAddress correspondence and reprint requests to: Dr Po-Liang Lu, Department of Internal Medicine, Kaohsiung Medical University Hospital, 100 Tzyou 1st Road, Kaohsiung 807, Taiwan

Received 18 April 2008; accepted 2 June 2008.

Article Outline

The genetic relationships among dengue virus serotype 2 (DEN-2) isolates from the Taiwan 2002 epidemic were studied by sequence analysis of the envelope (E) and nonstructural protein 1 (NS1) genes. A 0–0.4% divergence among 10 isolates revealed an epidemic strain in the outbreak. Phylogenetic study demonstrated that the 2002 Taiwan isolates were of the Cosmopolitan genotype, which is different from the Asian 1 and Asian 2 genotypes of Taiwan DEN-2 isolates from 1981 to 1998 and the American/Asian genotype of 2005 Taiwan isolates. Although grouping results from both E and NS1 gene sequence analyses were the same, the usage of the NS1 gene as a sequence analysis target has not been validated for the lower bootstrap support values of branches in the phylogenetic tree. Our result showing the same genotype changes in Taiwan and Philippines isolates suggests strain transfer of DEN-2 to nearby countries resulting in the same trend of genotype change.

Key Words:  dengue virus serotype 2 , envelope gene , nonstructural protein 1 gene

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PII: S1607-551X(08)70163-3

doi:10.1016/S1607-551X(08)70163-3

Kaohsiung Journal of Medical Sciences
Volume 24, Issue 8 , Pages 398-407, August 2008

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