Kaohsiung Journal of Medical Sciences
Volume 24, Issue 11 , Pages 577-590, November 2008

Differential CCR4 Expression And Function in Cutaneous T-Cell Lymphoma Cell Lines

  • Chieh-Shan Wu

      Affiliations

    • Department of Dermatology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taichung, Taiwan
    • ,
  • Sin-Ting Wang

      Affiliations

    • Department of Dermatology, China Medical University, Taichung, Taiwan
    • ,
  • Chai-Yu Liao

      Affiliations

    • Department of Dermatology, China Medical University, Taichung, Taiwan
    • ,
  • Meng-Tse Wu

      Affiliations

    • Department of Dermatology, China Medical University, Taichung, Taiwan
    • Corresponding Author InformationAddress correspondence and reprint requests to: Dr Meng-Tse Wu, Department of Dermatology, China Medical University, 2 Yuh Der Road, Taichung 404, Taiwan

Received 9 September 2008; accepted 18 November 2008.

Article Outline

Cutaneous T cell lymphoma (CTCL) is a clonal epidermotropic malignancy of memory T cells primarily involving the skin. However, the mechanisms governing migration of CTCL cells have not been fully clarified. It has been shown that certain chemokine receptors are upregulated in CTCL cells, but it remains unanswered whether these chemokine receptors play a critical role in the migration dynamics of CTCL. Using cell lines originally derived from patients with different subtypes of CTCL, we have shown higher CCR4 expression in the line derived from the mycosis fungoides (MJ), compared with the line derived from Sézary syndrome (Hut78). In specific responses to CCL22 (a CCR4 ligand) treatments, MJ cells showed significant chemotactic migration, enhanced activation and adhesion of certain integrins (CD49d and CD29) in vitro, while the control cells (Hut78, CD4+CD45RO+ memory T cells, and Jurkat cells) did not. Furthermore, compared with Hut78 cells, MJ cells manifested significantly more transendothelial migration in responses to treatments with either CCL22 or conditioned medium from dendritic cells in vitro. These results provide further dynamic evidence, in line with the multistep cascade paradigm for leukocyte transendothelial migration, to support a critical role for CCR4 in CTCL migration.

Key Words:  CCR4 , chemokine , cutaneous T-cell lymphoma

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PII: S1607-551X(09)70019-1

doi:10.1016/S1607-551X(09)70019-1

Kaohsiung Journal of Medical Sciences
Volume 24, Issue 11 , Pages 577-590, November 2008

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