Effects of Antiglaucoma Drugs on Calcium Mobility in Cultured Corneal Endothelial Cells

Wu, Kwou-Yeung; Hong, Show-Jen; Wang, Hwei-Zu

doi:10.1016/S1607-551X(09)70222-0

« Previous Next »Kaohsiung Journal of Medical Sciences
Volume 22, Issue 2 , Pages 60-67, February 2006

Effects of Antiglaucoma Drugs on Calcium Mobility in Cultured Corneal Endothelial Cells

Kwou-Yeung Wu
- Department of Ophthalmology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
- Department of Ophthalmology, Kaohsiung Municipal Hsiao-Kang Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan
Show-Jen Hong
- Department of Pharmacology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
Hwei-Zu Wang
- Department of Ophthalmology, Faculty of Medicine, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
- Department of Ophthalmology, Kaohsiung Medical University Chung-Ho Memorial Hospital, Kaohsiung Medical University, Kaohsiung, Taiwan
- Address correspondence and reprint requests to: Dr. Hwei-Zu Wang, Department of Ophthalmology, Kaohsiung Medical University, 100 Shih-Chuan 1^st Road, Kaohsiung, Taiwan

Received 19 August 2005; accepted 8 December 2005.

The aim of this study was to estimate the effects of various antiglaucoma drugs including betaxolol, timolol, levobunolol, brimonidine, carteolol, dipivefrin, dorzolamide, brinzolamide, latanoprost, unoprostone, and pilocarpine on intracellular free Ca²⁺ ([Ca²⁺]_i) mobility in cultured bovine corneal endothelial cells. Various antiglaucoma drugs were diluted from original concentrations to 1/100, 1/1,000, and 1/10,000. The [Ca²⁺]_i mobility was studied by spectrofluorophotometry after loading with the ester of fura-2 (fura-2/AM). It was found that timolol (58 μM and 5.8 μM), levobunolol (171 μM, 17.1 μM, and 1.71 μM), betaxolol (162 μM, 16.2 μM, and 1.62 μM), carteolol (680 μM and 68 μM), dipivefrin (28 μM and 2.8 μM), dorzolamide (616 μM and 61.6 μM), brinzolamide (260 μM), latanoprost (1.1 μM), unoprostone (28.2 μM, 2.82 μM, and 0.282 μM), and pilocarpine (408 μM and 40.8 μM) induced a significant increase in [Ca²⁺]_i. Nevertheless, only brimonidine (68 μM and 6.8 μM) decreased [Ca²⁺]_i concentration significantly. Benzalkonium chloride preservative did not affect [Ca²⁺]_i after addition of 0.001, 0.0001 and 0.00001 mg/mL to cells. These results indicate that all antiglaucoma drugs may affect the physiologic function of corneal endothelial cells through change of [Ca²⁺]_i.mobility.