Kaohsiung Journal of Medical Sciences
Volume 22, Issue 3 , Pages 143-148, March 2006

Polymerase Chain Reaction Identification of a Hymenopteran Insect in the Cornea: a Case Report

  • Wen-Bin Yeh

      Affiliations

    • Department of Entomology, National Chung Hsing University, Taichung, Taiwan
    • ,
  • Yaw-Syan Fu

      Affiliations

    • Faculty of Biomedical Science and Environment Biology, Kaohsiung Medical University, Kaohsiung, Taiwan
    • ,
  • Chien-Chung Cheng

      Affiliations

    • Faculty of Biomedical Science and Environment Biology, Kaohsiung Medical University, Kaohsiung, Taiwan

Department of Ophthalmology, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan

Received 14 September 2005; accepted 1 December 2005.

The type of corneal injuries associated with insect encounters is related to the composition of the foreign body. However, previous reports on corneal foreign bodies as insects were rarely based on scientific evidence. Here, we report on a 49-year-old male who was stung in his left eye by an unknown insect. Emergent keratotomy was performed to remove the embedded corneal foreign body. The removed foreign body was observed under light microscopy, and a fragment of insect was suspected. The sample was sent for molecular analysis. The polymerase chain reaction product was sequenced, subjected to a BLAST search, and identified as an ichneumonoid member of the insect order Hymenoptera.

Key Words:  corneal insect foreign body , polymerase chain reaction , Hymenoptera

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PII: S1607-551X(09)70234-7

doi:10.1016/S1607-551X(09)70234-7

Kaohsiung Journal of Medical Sciences
Volume 22, Issue 3 , Pages 143-148, March 2006

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