Volume 26, Issue 2, Pages 68-75 (February 2010)

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Toona Sinensis Extracts Induced Cell Cycle Arrest and Apoptosis in the Human Lung Large Cell Carcinoma

Received 21 April 2009; accepted 1 October 2009.

Toona sinensis extracts have been shown to exhibit anti-cancer effects in human ovarian cancer cell lines, human promyelocytic leukemia cells and human lung adenocarcinoma. Its safety has also been confirmed in animal studies. However, its anti-cancer properties in human lung large cell carcinoma have not been studied. Here, we used a powder obtained by freeze-drying the super-natant of centrifuged crude extract from Toona sinensis leaves (TSL-1) to treat the human lung carcinoma cell line H661. Cell viability was evaluated by the 3-(4-,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay. Flow cytometry analysis revealed that TSL-1 blocked H661 cell cycle progression. Western blot analysis showed decreased expression of cell cycle proteins that promote cell cycle progression, including cyclin-dependent kinase 4 and cyclin D1, and increased the expression of proteins that inhibit cell cycle progression, including p27. Furthermore, flow cytometry analysis showed that TSL-1 induced H661 cell apoptosis. Western blot analysis showed that TSL-1 reduced the expression of the anti-apoptotic protein B-cell lymphoma 2, and degraded the DNA repair protein, poly(ADP-ribose) polymerase. TSL-1 shows potential as a novel therapeutic agent or for use as an adjuvant for treating human lung large cell carcinoma.

Key Words: apoptosis , cell cycle , lung cell carcinoma , Toona sinensis

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References

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a Department of Internal Medicine, Kaohsiung Medical University Chung-Ho Memorial Hospital, Changhuan, Kaohsiung, Taiwan

b Department of Internal Medicine, Changhuan Christian Hospital, Changhuan, Kaohsiung, Taiwan

c Department of Physiology, Kaohsiung Medical University, Kaohsiung, Taiwan

d Department of Internal Medicine, Faculty of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

e Department of Respiratory Therapy, College of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan

Address correspondence and reprint requests to: Dr Ming-Shyan Huang, Department of Internal Medicine, Kaohsiung Medical University Chung-Ho Memorial Hospital, 100 Shih-Chuan 1st Road, Kaohsiung 807, Taiwan

* Cheng-Yuan Wang and Kai-Huang Lin contributed equally to this work.

PII: S1607-551X(10)70010-3

doi:10.1016/S1607-551X(10)70010-3

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